Human PSAT1 ELISA Kit (HUFI07019)

The Assay Genie Human PSAT1 ELISA Kit enables quantitative measurement of phosphoserine aminotransferase 1, a key enzyme in the L-serine biosynthesis pathway that converts 3-phosphohydroxypyruvate to phosphoserine. PSAT1 has attracted significant research interest as a metabolic marker in oncology, with elevated expression reported in breast carcinoma, non-small cell lung cancer, and colorectal adenocarcinoma tissues, where it supports de novo serine synthesis needed for rapid tumor proliferation.

This quantitative immunoassay runs on a pre-coated microplate and uses human-specific capture antibodies that exhibit no significant cross-reactivity with closely related aminotransferases such as PSAT2 or PSPH. Researchers can apply the workflow to liquid biopsy samples and cleared supernatant matrices to study cancer metabolism, serine-glycine pathway biology, or PSAT1 as a putative circulating biomarker. Each kit is configured for research use only and supports 96 determinations across the supplied well plate, including a serial-dilution standard curve for interpolating unknown concentrations in vitro.

For optimal reagent stability, store the sealed microplate and lyophilized standards refrigerated at 4 degrees C and protect from light; bring all components to room temperature before initiating the assay. Aliquot reconstituted standards to limit freeze-thaw cycles, and consult the lot-specific certificate of analysis for the exact sensitivity value, assay range, and recommended sample volume prior to first use.