Human PRSS23 ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the accurate quantification of human PRSS23 (Serine Protease 23) levels across a range of sample types, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The double antibody sandwich format uses a colorimetric detection method, with a reported sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL.

PRSS23 is a serine protease involved in inflammation, tissue remodeling, and immune response regulation, and its dysregulation has been linked to cancer, autoimmune diseases, and inflammatory disorders. The kit is positioned for research applications in these areas, under the research area of Epigenetics and Nuclear Signaling. Reported recovery values across serum, EDTA plasma, and heparin plasma are provided in the source documentation, alongside linearity data for the same matrices. The kit is intended for storage at 2-8°C for 12 months.

The assay workflow uses a pre-coated plate with biotin-labeled detection antibody and HRP-Streptavidin (SABC), with TMB substrate development and absorbance read at 450 nm. Key incubations are at 37°C for 90 minutes (primary), 60 minutes (detection antibody), and 30 minutes (HRP-Streptavidin), with TMB development carried out in the dark for 10-20 minutes.