Human PRPF40A ELISA Kit (HUFI07051)

The PRPF40A target protein (Pre-mRNA Processing Factor 40 Homolog A) participates in constitutive and alternative pre-mRNA splicing as part of the U1 small nuclear ribonucleoprotein complex. Its tandem WW and FF repeat domains mediate protein-protein interactions that recruit downstream spliceosome components to nascent transcripts, and altered PRPF40A expression has been documented in spliceosome-related disease models.

Researchers in RNA biology, neurodegeneration studies, and cancer biomarker discovery use this quantitative immunoassay from Assay Genie to measure PRPF40A concentrations in human-derived samples. The microplate-based workflow accommodates standard curves and replicate wells for parallel cohort analyses, with all capture antibodies, detection reagents, and calibration standards provided in a single kit format.

Reconstitute lyophilized components per the enclosed protocol, equilibrate reagents to room temperature before initiating the assay, aliquot working solutions to minimise freeze-thaw cycles, and refrigerate the kit between experiments to preserve antibody binding activity.