Human PRPF38A ELISA Kit (HUFI07047)

PRPF38A is a core component of the human spliceosome, participating in the assembly of the U4/U6.U5 tri-snRNP and in catalytic step 2 of pre-mRNA splicing. The Assay Genie PRPF38A ELISA Kit provides a quantitative immunoassay workflow for measuring Pre-mRNA Processing Factor 38A levels in research samples, supporting studies of RNA processing, alternative splicing regulation, and cellular mechanisms in which spliceosomal function is disrupted.

Researchers in molecular biology and gene expression fields apply this kit to monitor PRPF38A abundance in cell-based models, to track changes following RNAi knockdown, CRISPR editing, or pharmacological treatment, and to compare expression across tissue or cell-line panels. The in vitro ELISA workflow is intended for research use only and follows a standard microplate protocol that fits into batched sample processing on conventional laboratory plate readers.

Assay Genie supplies the kit as a complete reagent set, and the procedure is compatible with standard ELISA instrumentation and liquid-handling workflows. PRPF38A readouts complement orthogonal methods such as western blot and qPCR, and they are useful in screens that probe spliceosome integrity under stress, transcriptional perturbation, or compound exposure. Results support downstream analysis of splicing factor stoichiometry and characterization of disease-relevant splicing phenotypes.