Human Protoporphyrinogen Oxidase (PPOX) ELISA Kit

For research into human heme biosynthesis, the Assay Genie protoporphyrinogen oxidase immunoassay kit quantifies PPOX levels in tissue homogenates and cell lysate preparations. PPOX resides on the inner mitochondrial membrane and catalyzes the final oxidation step from protoporphyrinogen IX to protoporphyrin IX, supporting investigations into mitochondrial enzyme regulation, porphyria pathophysiology, and hepatic heme metabolism.

Each kit contains pre-coated microplate wells and reagents for 96 assays, with an absorbance-based endpoint readable on standard plate readers. The protocol serves exploratory biomarker profiling, longitudinal studies of PPOX expression in disease models, and validation of results obtained from complementary methods including western blot, PCR, and immunohistochemistry.

Scientists studying variegate porphyria, drug-induced hepatotoxicity affecting heme synthesis, or erythroid differentiation can incorporate this kit into broader workflows involving cell culture and in vitro model systems.