Human Protocadherin alpha-1 (PCDHA1) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the quantitative detection of human Protocadherin alpha-1 (PCDHA1), also known as PCDH-alpha-1. It is intended for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, and uses a colorimetric detection method. The kit is reactive toward natural and recombinant human Protocadherin alpha-1.

Reported performance specifications include a detection range of 0.625-40ng/mL, a sensitivity of 0.33ng/mL, an intra-assay CV of 9.4% and an inter-assay CV of 10.1%. Dilution linearity for serum (N=5) spans 85-115% across 1:2 to 1:16 dilutions, with EDTA plasma spanning 83-122% and heparin plasma spanning 86-115%. Recovery averages 94% (88-100%) for serum and 96% (90-102%) for plasma.

PCDHA1 is described as a potential calcium-dependent cell-adhesion protein that may be involved in the establishment and maintenance of specific neuronal connections in the brain, with isoform 2 reported as secreted. The associated UniProt entry is Q9Y5I3 and the protein has a molecular weight of 87,639 Da. For research use only.