Human Proto-oncogene Wnt-1 (WNT1) ELISA Kit

The WNT1 protein is a proto-oncogene that plays a key role in regulating cell growth and development, with dysregulation linked to various cancers including breast and colorectal cancer, making it a valuable biomarker for studying tumorigenesis and potential therapeutic interventions. This sandwich ELISA kit from Assay Genie is designed to measure levels of the WNT1 protein in human serum, plasma, and cell culture supernatants, with reactivity specific to human samples.

Performance characteristics include a detection range of 12.5-800pg/mL and sensitivity of 9.8pg/mL, with an intra-assay CV of 3.7% and inter-assay CV of 7.9%. Specificity is directed against natural and recombinant human Proto-oncogene Wnt-1. Recovery testing shows averages of 85% (range 80-91%) for serum and 87% (range 81-93%) for plasma. Linearity data across dilutions of 1:2, 1:4, 1:8, and 1:16 demonstrates robust performance in serum, EDTA plasma, and heparin plasma samples. Acceptable sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

WNT1 functions as a ligand for members of the frizzled family of seven transmembrane receptors and, in some developmental processes, also serves as a ligand for the coreceptor RYK, triggering Wnt signaling. It is classified as a probable developmental protein that may act as a signaling molecule in CNS development, with additional roles in osteoblast function and bone development. The kit is intended for research use only and supports cancer research applications. The associated UniProt entry is P04628.