Human Protein Wnt-3a (WNT3A) ELISA Kit

Designed for the precise measurement of WNT-3A levels in human samples, this Sandwich ELISA Kit from Assay Genie is validated for serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. The colorimetric assay offers specificity for natural and recombinant human Protein Wnt-3a and is intended for research use only, with a research area of cancer.

Performance characteristics include a detection range of 0.156-10ng/mL and a sensitivity of 0.067ng/mL. The kit reports an intra-assay CV of 5.4% and an inter-assay CV of 7.7%. Linearity has been demonstrated across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16. Recovery averages 90% for serum (range 84-96%) and 92% for plasma (range 86-98%).

WNT-3A is a secreted signaling protein that functions as a ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Dysregulation of WNT-3A has been linked to multiple diseases, including cancer, osteoporosis, and developmental disorders, providing context for research into these areas.