Human Protein SAAL1 (SAAL1) ELISA Kit

From Assay Genie, this sandwich ELISA kit with colorimetric detection is designed for the quantitative measurement of human Protein SAAL1 (also known as Synoviocyte proliferation-associated in collagen-induced arthritis protein 1 or SPACIA1) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. SAAL1 is a member of the serum amyloid A (SAA) family, and elevated levels have been linked to inflammatory conditions, making the protein a useful biomarker for studying inflammatory diseases and potential therapeutic interventions. According to UniProt entry Q96ER3, the protein has a molecular weight of 53,558 Da, is encoded at chromosomal location 11p15.1, and plays a role in promoting the proliferation of synovial fibroblasts in response to proinflammatory stimuli.

The assay reports a detection range of 0.156–10 ng/mL with a sensitivity of 0.056 ng/mL. Intra-assay CV is 6.5% and inter-assay CV is 9.9%. Linearity has been demonstrated in serum (85–93% at 1:2, 98–109% at 1:4, 102–110% at 1:8, 82–91% at 1:16), EDTA plasma (100–110% at 1:2, 82–91% at 1:4, 112–122% at 1:8, 96–105% at 1:16) and heparin plasma (107–117% at 1:2, 87–96% at 1:4, 92–104% at 1:8, 100–110% at 1:16). Spike-and-recovery values average 106% for serum (range 100–112%) and 108% for plasma (range 102–114%). The kit is specific for natural and recombinant human Protein SAAL1 and is intended for research use only.