Human Protein phosphatase methylesterase 1 (PPME1) ELISA Kit

From Assay Genie, this competitive ELISA kit is designed for the quantitative detection of human Protein phosphatase methylesterase 1 (PPME1) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The colorimetric assay is reactive with human samples and provides high specificity for natural and recombinant human PPME1.

Performance specifications include a detection range of 0.625-40ng/mL, a sensitivity of 0.324ng/mL, an intra-assay CV of 6.3% and an inter-assay CV of 7.6%. The kit supports research into PPME1, an enzyme involved in the regulation of protein methylation and dephosphorylation processes that play a crucial role in maintaining cellular homeostasis and signaling pathways.

Functionally, PPME1 demethylates proteins that have been reversibly carboxymethylated, including PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme. The protein belongs to the AB hydrolase superfamily, has a molecular weight of 43,870 Da and is catalogued under UniProt Q9Y570. Intended for research use only.