Human Protein LYRIC (MTDH) ELISA Kit

From Assay Genie, this Sandwich ELISA is intended for the quantitative measurement of human Protein LYRIC (MTDH) (Metadherin) in human samples. The assay is reactive with human samples, with reported uses covering serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, and is described as specific for natural and recombinant human Protein LYRIC.

The reported detection range is 1.56-100nmol/L with a sensitivity of 0.092nmol/L. Reported precision values include an Intra CV of 3.2% and an Inter CV of 6.5%. Linearity is reported as: Serum (N=5) 102-111% / 80-90% / 88-98% / 96-108%; EDTA Plasma (N=5) 105-115% / 89-99% / 107-117% / 96-107%; Heparin Plasma (N=5) 89-99% / 116-128% / 91-99% / 101-109% (at dilutions 1:2, 1:4, 1:8, 1:16 respectively). Reported recovery is Serum average 104% (range 98-110%) and Plasma average 106% (range 100-112%). Synonyms listed include 3D3/LYRIC, Astrocyte elevated gene-1 protein, Lysine-rich CEACAM1 co-isolated protein, Metadherin, Metastasis adhesion protein, AEG-1, AEG1 and LYRIC. UniProt: Q86UE4. NCBI Full Name: Metadherin. Molecular Weight: 63,837 Da.

The kit is for research use only, and the protein is described as promoting anchorage-independent growth, lung metastasis and chemoresistance, and as activating NF-kappa-B transcription factor. Reported subcellular localisation includes endoplasmic reticulum membrane, nucleus membrane, tight junction, nucleolus, cytoplasm and perinuclear region; TNF-alpha causes translocation from the cytoplasm to the nucleus. The research area is given as Epigenetics, with reported interactions with BCCIP, CREBBP/CBP and RELA/p65.