Human Protein lin-28 homolog A (LIN28A) ELISA Kit

From Assay Genie, this Sandwich ELISA kit uses colorimetric detection and is designed for the precise measurement of human Protein lin-28 homolog A (LIN28A) in serum, plasma, and cell culture supernatants, and is also suitable for tissue homogenates and other biological fluids. It is reactive with human samples and detects both natural and recombinant forms of the target.

Manufacturer data report a detection range of 0.156-10 ng/ml and a sensitivity of 0.089 ng/mL. Reported precision values are an intra-assay CV of 4.6% and an inter-assay CV of 8.1%. The assay is intended for research use only, with applications in stem cell research.

LIN28A is an RNA-binding protein that acts as a translational enhancer, driving specific mRNAs to polysomes and regulating translation of mRNAs that control developmental timing, pluripotency, and metabolism. It inhibits processing of pre-let-7 miRNAs and is involved in stem cell differentiation, tissue development, and cancer initiation. The protein is predominantly cytoplasmic but shuttles between the cytoplasm and the nucleus, with reported localization to the cytoplasm, nucleus, nucleolus, rough endoplasmic reticulum, cytoplasmic processing bodies, and stress granules. Listed synonyms include Zinc finger CCHC domain-containing protein 1, Lin-28A, CSDD1, LIN28, and ZCCHC1 (UniProt: Q9H9Z2).