Human Protein Kinase B Alpha (PKBa) ELISA Kit

Researchers working on the PI3K/Akt signaling pathway can use this colorimetric ELISA kit from Assay Genie to quantify Protein Kinase B Alpha (PKBa/Akt1) levels in human samples. The assay detects endogenous PKBa in tissue homogenates and cell lysates, supporting studies of the signaling cascade that governs cell survival, proliferation, and metabolic responses. Akt1 dysregulation features prominently in many cancers and metabolic disorders, making quantitative monitoring of this kinase valuable across oncology, diabetes, and signal transduction research. Each kit contains reagents for 96 assays with a colorimetric readout that works on standard microplate readers. The platform handles cell line models and primary tissue specimens, enabling comparison of PKBa expression across experimental conditions, treatment timepoints, or genetic backgrounds. Sample preparation typically involves homogenization or lysis in standard buffers, after which the workflow follows familiar immunoassay protocols used in most molecular biology laboratories.