Human Protein Kinase, AMP Activated Alpha 1 (PRKAa1) ELISA Kit

PRKAA1 (AMPKα1) encodes the catalytic α1 subunit of AMP-activated protein kinase, a heterotrimeric serine/threonine kinase that serves as a central regulator of cellular energy balance across human tissues. This Assay Genie ELISA kit delivers quantitative measurement of AMPKα1 protein abundance in research samples including tissue homogenates and cell lysates, supporting studies in metabolism, oncology, exercise physiology, and pharmacology.

The AMPKα1 catalytic subunit has a molecular mass of approximately 63 kDa and is expressed in most human cell types, where it senses cellular AMP:ATP ratios and metabolic stress to phosphorylate downstream targets that govern glucose uptake, fatty acid oxidation, mitochondrial biogenesis, and autophagy. Researchers use AMPKα1 quantification to assess activation by pharmacological compounds such as metformin, AICAR, and resveratrol, to distinguish isoform-specific contributions from the PRKAA2-encoded AMPKα2 subunit that dominates in skeletal and cardiac muscle, and to profile pathway status in disease models spanning insulin resistance, type 2 diabetes, obesity, and tumor bioenergetics.

Each microplate-based kit supports 96 determinations using an immunoassay workflow with colorimetric signal readout compatible with standard laboratory plate readers. The assay accommodates the sample types listed in the product specifications and is intended for research use only.