Human Protein fem-1 homolog A (FEM1A) ELISA Kit

Manufactured by Assay Genie, this sandwich-format immunoassay is designed for the quantitative measurement of Human Protein fem-1 homolog A (FEM1A). The kit uses a colorimetric detection method and is reactive with human samples, with documented specificity for both natural and recombinant human Protein fem-1 homolog A.

The assay is validated for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Reported performance characteristics include a detection range of 0.312-20ng/mL, a sensitivity of 0.16ng/mL, an intra-assay CV of 3.6% and an inter-assay CV of 6.2%. Linearity in serum, EDTA plasma and heparin plasma is documented across dilution series from 1:2 to 1:16, and recovery ranges of 80-91% for serum and 81-93% for plasma have been reported. FEM1A is localised to the cytoplasm, and the kit is supplied with reagents for research use only.

FEM1A is a probable component of an E3 ubiquitin-protein ligase complex, where it may act as a substrate recognition subunit, and may also participate in antiinflammatory signaling through interaction with PTGER4. The protein is associated with biological processes including regulation of ubiquitin-protein ligase activity, negative regulation of inflammatory response and protein ubiquitination, with reported molecular functions of EP4 subtype prostaglandin E2 receptor binding and ubiquitin-protein ligase activity. Synonyms referenced for the target include FEM1-alpha, FEM1a, Prostaglandin E receptor 4-associated protein and EPRAP.