Human Proteasome Activator Subunit 3 (PSME3) ELISA Kit

The PSME3 target, also designated REGγ or PA28γ, is an 11S family proteasome activator that drives ATP- and ubiquitin-independent degradation of regulatory proteins through the 20S core, including cell cycle and apoptosis regulators such as p21, p16, and MDM2. Researchers rely on this Assay Genie kit to quantify PSME3 across tumor and normal tissue contexts, with particular relevance to MDM2-p53 pathway studies in hepatocellular, colorectal, and breast cancer models. Each pack contains a pre-coated microplate strip plate, lyophilized standards, biotinylated detection antibody, and streptavidin-HRP conjugate; reconstituted components should be aliquoted and stored frozen to limit freeze-thaw cycles between runs. The human-specific capture antibody is paired with a biotinylated detection reagent to minimize cross-reactivity with homologous REG subunits. Intended for in vitro research use only, the kit supports a single-use 96-well microplate workflow with standards and unknowns loaded in parallel and absorbance read on a standard plate reader.