Human Prostate-specific antigen (KLK3) ELISA Kit

Designed for the quantitative measurement of human prostate-specific antigen (KLK3), this Sandwich ELISA kit from Assay Genie targets KLK3, also known by synonyms including PSA, Kallikrein-3, Gamma-seminoprotein, Seminin, Semenogelase, P-30 antigen, and APS. The assay is reactive with human samples and is intended for research use only.

Validated sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, with specificity for natural and recombinant human Prostate-specific antigen. Reported performance characteristics are a detection range of 0.156-10ng/mL and a sensitivity of 0.078ng/mL. Precision values are 4.6% intra-assay CV and 9.5% inter-assay CV. Recovery is documented in serum (average 100%, range 94-106%) and plasma (average 102%, range 96-108%), and linearity has been evaluated across 1:2, 1:4, 1:8, and 1:16 dilutions for serum, EDTA plasma, and heparin plasma.

According to the underlying UniProt record (P07288), KLK3 hydrolyzes semenogelin-1, contributing to the liquefaction of the seminal coagulum, and forms a heterodimer with SERPINA5. The protein is a secreted serine protease (EC 3.4.21.77) of the kallikrein subfamily, mapped to chromosome 19q13.41, and is relevant to cancer research, particularly prostate cancer biomarker studies.