Human Prosaposin (PSAP) ELISA Kit

Assay Genie's quantitative immunoassay for human prosaposin (PSAP) enables researchers to measure this neurotrophic factor precursor across many specimen types. The kit, supplied in a 96-assay configuration, is intended for in vitro research use only and supports work in lysosomal storage research, sphingolipid metabolism, and neurodegeneration studies where PSAP serves as a candidate biomarker.

Compatible human samples include patient serum, donor plasma, tissue homogenates, cell lysates, cerebrospinal fluid, seminal plasma, and cell culture supernatants, giving laboratories flexibility for both clinical specimen banking and cell-based experimentation. Detection relies on an enzymatic reaction that produces a measurable color change, read on a standard microplate reader. Each kit contains a pre-coated plate, standards, and reagents sufficient for 96 determinations, allowing full plate runs or smaller batched experiments.

Prosaposin is processed intracellularly into saposins A, B, C, and D, small activator proteins that stimulate lysosomal hydrolases involved in glycosphingolipid breakdown, making accurate quantification valuable for studies of Gaucher disease, Krabbe disease, and metachromatic leukodystrophy. The Assay Genie protocol emphasizes straightforward liquid handling with no specialized equipment beyond a standard plate washer and reader, accessible to most research laboratories working on lysosomal biology.