Human Proprotein convertase subtilisin/kexin type 9 (PCSK9) ELISA Kit

A sandwich ELISA kit from Assay Genie is designed for the precise measurement of Proprotein convertase subtilisin/kexin type 9 (PCSK9) levels in human serum, plasma, and cell culture supernatants. The target is the human protein, also referenced by synonyms including Neural apoptosis-regulated convertase 1, Proprotein convertase 9, Subtilisin/kexin-like protease PC9, NARC-1, PC9, PSEC0052, and NARC1.

Detection is by colorimetric ELISA, and the assay is intended for research use only. Reported performance characteristics include a detection range of 0.312-20ng/mL, a sensitivity of 0.12ng/mL, an intra-assay CV of 5.0%, and an inter-assay CV of 8.1%. Linearity has been assessed in serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16, and recovery data report averages of 99% for serum (93-105) and 101% for plasma (95-107).

PCSK9 is described as a crucial player in plasma cholesterol homeostasis, binding low-density lipoprotein receptor family members LDLR, VLDLR, LRP1/APOER, and LRP8/APOER2 and promoting their degradation in intracellular acidic compartments. Dysregulation of PCSK9 has been linked to cardiovascular diseases, positioning the kit as a tool for biomarker and cardiovascular research.