Human Prolyl 4-hydroxylase subunit alpha-2/P4HA2 ELISA Kit

A high-quality Sandwich ELISA for the quantitative detection of Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) in human samples, manufactured by Assay Genie. The kit uses a double-antibody sandwich format with a colorimetric readout and is positioned for research in cell biology, including studies of collagen metabolism, fibrotic diseases, cancer and connective tissue disorders.

The assay has a sensitivity of 0.469 ng/mL and a working range of 0.781–50 ng/mL. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples; the kit is stored at 2–8 °C for 12 months. The procedure involves primary incubation at 37 °C for 90 minutes, biotinylated detection antibody incubation for 60 minutes, HRP-Streptavidin binding for 30 minutes, TMB color development for 10–20 minutes, and absorbance measurement at 450 nm.

Reported linearity spans 80–105% across 1:2 to 1:8 dilutions for serum, EDTA plasma and heparin plasma (n = 5). Average recovery is 98% in serum, 97% in EDTA plasma and 96% in heparin plasma. P4HA2 is a key enzyme in collagen hydroxylation, and the kit is described as a reliable tool for quantifying P4HA2 levels in human samples for a variety of research applications.