Human Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) ELISA Kit

Quantify the P4Ha3 collagen-processing enzyme in human tissue samples with this colorimetric immunoassay kit from Assay Genie. Prolyl-4-hydroxylase alpha polypeptide III is one of three alpha subunit isoforms of prolyl 4-hydroxylase, the enzyme complex responsible for hydroxylation of proline residues during collagen biosynthesis. Measurement of P4Ha3 levels supports research into extracellular matrix remodeling, fibrotic tissue changes, and tumor microenvironments where collagen turnover is altered.

Supplied as a complete kit sufficient for 96 determinations, the assay relies on a colorimetric readout compatible with standard microplate readers. Protocols are intended for laboratory research use only, with sample preparation from tissue homogenates following standard homogenization and centrifugation steps. Quantitative results are interpreted against a standard curve generated from included reference standards, enabling sensitive detection of P4Ha3 across experimental conditions.