Human Prolyl-4-Hydroxylase Alpha Polypeptide II (P4Ha2) ELISA Kit

As a critical enzyme in collagen biosynthesis, Prolyl-4-Hydroxylase Alpha Polypeptide II (P4HA2) catalyzes post-translational hydroxylation of proline residues required for proper procollagen triple-helix formation and collagen fiber stability. This colorimetric ELISA from Assay Genie is validated for quantitative measurement of human P4HA2 in tissue homogenates and supports research into collagen metabolism, fibrotic disease mechanisms, tumor microenvironment biology, and connective tissue disorders.

The kit is supplied in a 96 assays format with pre-coated plate strips, detection antibody, standards, and substrate reagent sufficient for the full plate run. Researchers investigating extracellular matrix remodeling in hepatic, pulmonary, or renal fibrosis, P4HA2 expression in breast and lung cancer models, and cartilage or bone metabolism will find this assay applicable for tracking P4HA2 protein levels across cell lysates and tissue extracts. The protocol uses standard ELISA plate readers, and the enzymatic reaction produces an absorbance change proportional to P4HA2 concentration in each well.

For research use only, this assay supports in vitro investigation of proline hydroxylation pathways and downstream effects on collagen crosslinking and stability. Reagents should be stored refrigerated between uses to preserve activity through the kit's shelf life, and the enclosed protocol outlines recommended sample dilution ranges, incubation times, and assay procedures for reproducible results across experiments.