Human Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) ELISA Kit

Prolyl 4-hydroxylase alpha-1 subunit (P4HA1) is the catalytic alpha component of the prolyl 4-hydroxylase tetramer responsible for hydroxylating proline residues during collagen biosynthesis. This colorimetric ELISA Kit from Assay Genie provides quantitative measurement of P4HA1 protein in human research samples, supporting investigations into fibrosis, extracellular matrix remodeling, and hypoxia-driven gene regulation.

The assay is configured in a 96 assays format and accepts tissue homogenates as input material, enabling protein expression profiling from cell lysates and dissected tissue preparations. P4HA1 expression has been linked to collagen deposition in fibrotic disease and to tumor progression under low-oxygen conditions, making the kit a useful tool for connective tissue biology, wound healing research, and oncology laboratories.

Key features include a colorimetric read-out on standard microplate readers, a reactivity profile specific to human P4HA1 protein for translational studies, and a complete workflow containing pre-coated wells, detection antibodies, and the buffer reagents required to run the plate.