Human Prokineticin Receptor 2 (PKR2) ELISA Kit

The Assay Genie ELISA Kit quantifies human prokineticin receptor 2 (PROKR2/PKR2) in tissue homogenates, supporting research into G-protein-coupled receptor signaling in reproductive and neuroendocrine contexts. Mutations in PROKR2 have been linked to Kallmann syndrome and normosmic idiopathic hypogonadotropic hypogonadism, so reproducible receptor-level measurement is valuable for investigations of GnRH neuron migration, olfactory bulb development, and angiogenic signaling pathways.

The assay uses a colorimetric readout compatible with standard microplate readers and includes reagents sufficient for 96 assays per kit. Sample preparation typically involves homogenizing fresh or flash-frozen tissue in an appropriate lysis buffer, with dilutions optimized to fall within the assay's working range. Researchers apply this format to characterize PROKR2 expression in hypothalamic tissue, gonadal extracts, and cell culture models probing prokineticin-2 signaling.

For reliable results, store unopened components refrigerated at 4 °C, protect the substrate solution from light, and allow plates to equilibrate to room temperature before use. The kit is supplied for research use only and is not intended for diagnostic procedures.