Human Prohibitin (PHB) ELISA Kit

Prohibitin (PHB) is a ubiquitously expressed, highly conserved 32 kDa mitochondrial chaperone localized to the inner mitochondrial membrane, where it supports respiratory chain assembly and stabilizes newly synthesized proteins. The Assay Genie kit enables quantitative measurement of PHB levels for investigations into mitochondrial dysfunction, cellular senescence, apoptosis regulation, and tumor biology, where altered PHB expression has been reported in breast, prostate, gastric, and colorectal cancer studies. The microplate format supports biomarker discovery and pathway studies in human cell biology, with results quantified in concentration units against a standard curve generated with each run. Reconstituted reagents and working standards should be aliquoted to limit freeze-thaw cycles, while unopened components are stored refrigerated until first use. This ELISA kit is intended for in vitro research use only, providing reproducible detection across multiple human specimen matrices including tissue homogenates and cell lysates.