Human Profilin-1 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for detecting and quantifying profilin-1 levels in human samples, supporting research into the protein's role as a key regulator of actin polymerization and cytoskeletal dynamics, with relevance to cell motility, division, and intracellular signaling pathways. The kit is configured for human reactivity and uses a colorimetric detection method.

The kit accepts a range of sample types, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is stored at 2-8°C for 12 months. Reported performance includes a sensitivity of 46.875 pg/mL and an assay range of 78.125-5000 pg/mL, using a sandwich double-antibody methodology with a TMB substrate workflow in which absorbance is read at 450 nm.

Linearity across serum, EDTA plasma and heparin plasma (n=5) at 1:2, 1:4 and 1:8 dilutions falls within 80-105%, and recovery averages 98% for serum, 94% for EDTA plasma and 97% for heparin plasma. The associated research area is cell biology, with the target linked to investigations of cancer, autoimmune disorders and neurological conditions associated with profilin-1 dysregulation.