Human Procollagen III (PCIII) ELISA Kit

An immunoassay from Assay Genie that quantifies human Procollagen III (PCIII), the N-terminal propeptide released during type III collagen biosynthesis. Circulating PIIINP concentrations rise with active type III collagen deposition, supporting hepatic fibrosis research, wound-healing models, pulmonary fibrosis studies, scleroderma investigations, and broader extracellular matrix remodeling projects.

The kit provides a microplate pre-coated with capture antibody, paired detection antibody, lyophilized reference standards, and chromogenic substrate reagents required to interpolate unknown values against a standard curve. Each plate supports parallel analysis of human donor samples and tissue homogenate preparations from translational cohorts or animal-model readouts, and the included protocol walks users through reagent preparation, incubation timing, and absorbance-based endpoint measurement on a standard plate reader.

Beyond liver fibrosis monitoring, Procollagen III quantification is applied in cardiac remodeling studies, dermal scarring research, and connective tissue disorder investigations. Assay Genie ships each kit with a lot-specific certificate of analysis, and the supplied protocol specifies recommended sample dilution ranges, wash conditions, and calculation methods for converting raw optical density readings into analyte concentrations.