Human Procollagen II C-Terminal Propeptide (PIICP) ELISA Kit

The PIICP (Procollagen II C-Terminal Propeptide) ELISA quantifies the C-terminal propeptide cleaved from procollagen II during fibril formation, providing a direct read-out of newly synthesized type II collagen rather than its breakdown products. Because type II collagen is the dominant matrix protein of hyaline cartilage, circulating PIICP levels are widely used to monitor chondrocyte biosynthetic activity, growth plate dynamics, and cartilage repair responses in orthopedic and connective tissue research.

Common research applications include tracking disease progression in osteoarthritis cohorts, evaluating chondrogenic differentiation of mesenchymal stem cells, profiling biomarkers in skeletal dysplasia studies, and assessing cartilage anabolism in in vitro and ex vivo explant models. The kit from Assay Genie ships with a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated standards spanning a working range of roughly 0.3–30 ng/mL, and buffered wash and substrate solutions; the assay uses 50 µL of sample per well and has been validated for human serum and plasma. All components should be refrigerated on arrival and protected from light to preserve antibody activity across repeated freeze-thaw cycles.