Human Probable 4-hydroxy-2-oxoglutarate aldolase, mitochondrial (HOGA1) ELISA Kit

Assay Genie provides a Sandwich ELISA Kit for the quantitative measurement of human Probable 4-hydroxy-2-oxoglutarate aldolase, mitochondrial (HOGA1). The kit is intended for use with human samples and accepts serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The reported detection range is 0.312-20ng/mL with a sensitivity of 0.1ng/mL, and precision is characterised by an intra-assay CV of 5.3% and an inter-assay CV of 10.1%.

Specificity is directed against natural and recombinant human 4-hydroxy-2-oxoglutarate aldolase, mitochondrial. Recovery in serum averages 100% (range 94-106%) and in plasma averages 102% (range 96-108%). Linearity has been established in serum, EDTA plasma and heparin plasma across dilutions from 1:2 to 1:16.

HOGA1 is a mitochondrial enzyme with a critical role in the metabolism of 4-hydroxy-2-oxoglutarate, a key intermediate in the TCA cycle, and it catalyses the final step in the metabolic pathway of hydroxyproline. Dysregulation of HOGA1 has been implicated in various metabolic disorders and diseases, making the target a relevant biomarker in research on mitochondrial function and metabolism. The protein functions as a homotetramer localised to the mitochondrion. For research use only.