Human Pro-Gastrin Releasing Peptide ELISA Kit

A Sandwich ELISA from Assay Genie for the quantitative measurement of human pro-gastrin releasing peptide in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a colorimetric, double antibody format suitable for research applications investigating this biomarker.

Performance specifications include a sensitivity of 0.938 pg/mL and a working range of 1.563-100 pg/mL. Linearity has been characterised for serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 sample dilutions, with corresponding recovery data reported across these matrices. The kit should be stored at 2-8°C for 12 months. The workflow involves equilibrating reagents, loading standards and samples for primary incubation, binding with a biotin-labeled detection antibody followed by HRP-streptavidin, colour development with TMB substrate, and absorbance reading at 450 nm.

Pro-gastrin releasing peptide is described in the product literature as a key biomarker associated with certain cancers, particularly small cell lung cancer, supporting research into disease progression, prognosis, and treatment efficacy.