Human PRMT8 ELISA Kit (HUFI07042)

Quantitative measurement of human protein arginine methyltransferase 8 in clinical and research samples is the core use case for this 96 Assays ELISA kit from Assay Genie, supporting neuroscience, neuro-oncology, and neurological disease biomarker workflows. PRMT8 is a neural-restricted type I arginine methyltransferase that catalyzes asymmetric dimethylation of arginine residues on substrate proteins and is predominantly expressed in the central nervous system, where it modulates ion channel function and motor neuron biology.

Investigators apply the kit to profile PRMT8 levels in human serum specimens and matched plasma samples drawn from clinical cohorts, to characterize methyltransferase behavior in glioblastoma-derived cellular models, and to screen candidate small-molecule modulators of arginine methylation. The immunoassay pairs a capture antibody specific to human PRMT8 with a biotinylated reporter antibody and a streptavidin-HRP conjugate, yielding an absorbance readout proportional to target concentration in each well.

The kit is supplied as a single-use microplate in strip-well format together with lyophilized and liquid reference standards spanning the working range, a biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, chromogenic substrate, and stop solution. Unopened components are kept refrigerated; the sealed plate pouch should be allowed to equilibrate to room temperature before opening to prevent condensation on the strip wells, and the HRP conjugate is protected from prolonged light exposure to preserve enzymatic activity. Absorbance is captured on a standard microplate reader, and quantitative values are back-calculated against the standard curve run on the same plate.