Human PRKX ELISA Kit (HUFI07050)

PRKX (Protein Kinase X-linked) is a serine/threonine kinase encoded on the X chromosome. It participates in renal epithelial morphogenesis, spermatogenesis, and cAMP-dependent signal transduction, making it a target of interest in nephrology, reproductive biology, and intracellular signaling research. This kit from Assay Genie provides a quantitative immunoassay workflow for measuring endogenous PRKX protein in human blood-derived specimens.

Assay workflow uses a pre-coated microplate with paired capture and detection antibodies, a streptavidin-HRP conjugate, and a TMB substrate for an absorbance-based readout. Each run is calibrated against a recombinant PRKX standard curve, and results are interpolated to determine sample concentration. The kit is supplied with sufficient reagents for one plate of unknowns, standards, and controls.

Researchers commonly apply this assay in studies of X-linked kinase signaling, biomarker validation in clinical cohorts, and characterization of cAMP-driven pathways in disease models. Reagents should be stored refrigerated, protected from light, and equilibrated to room temperature before use to ensure consistent signal development across the plate.