Human PRKAR1A (Protein kinase cAMP-dependent type I regulatory subunit alpha) ELISA Kit (HUFI07059)

The PRKAR1A protein serves as the primary regulatory subunit of cyclic-AMP dependent protein kinase A (PKA), a central transducer of cAMP signaling in endocrine, cardiac and adipose tissues. This Assay Genie immunoassay supports research into PKA pathway dysregulation, Carney complex, primary pigmented nodular adrenocortical disease (PPNAD) and cAMP-mediated tumor biology. The microplate kit is calibrated against full-length recombinant human PRKAR1A and reports quantitative levels appropriate for comparative studies of cAMP pathway activity. Researchers typically load 100 uL of prepared sample per well and read absorbance on a standard plate reader after incubation steps at 37 degrees C. Unused strips should be resealed with desiccant and stored refrigerated at 4 degrees C, protected from light; reconstituted reagents should be aliquoted to limit freeze-thaw cycles. For research use only in laboratory investigation of signal transduction, endocrine disorders and tumor marker studies.