Human PRKAB1/5'-AMP-activated kinase subunit beta-1 ELISA Kit

Designed by Assay Genie for the quantitative measurement of human PRKAB1 (5'-AMP-activated kinase subunit beta-1), this Sandwich ELISA employs a double-antibody, colorimetric detection format and delivers a sensitivity of 0.188 ng/mL across an assay range of 0.313–20 ng/mL. The kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is stored at 2–8°C for 12 months.

PRKAB1 is a key subunit of the AMP-activated protein kinase (AMPK) complex, which plays a crucial role in regulating cellular energy homeostasis and is involved in glucose uptake, lipid metabolism, and autophagy. Dysregulation of PRKAB1 has been linked to obesity, diabetes, and cancer, making it a valuable biomarker for metabolism research. The kit is supplied with a dismountable ELISA microplate, lyophilized standard, biotin-labelled detection antibody, HRP-Streptavidin conjugate (SABC, 100X), TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, wash buffer (25X), plate sealers, and a technical manual.

Reported linearity across 1:2, 1:4, and 1:8 dilutions spans 84–101% for serum, 80–103% for EDTA plasma, and 81–100% for heparin plasma. Spike-and-recovery averages are 93% in serum, 97% in EDTA plasma, and 99% in heparin plasma. The assay procedure involves a 90-minute primary incubation at 37°C, a 60-minute biotinylated detection antibody step, a 30-minute HRP-Streptavidin binding step, TMB colour development in the dark for 10–20 minutes, and absorbance reading at 450 nm after stop solution addition.