Human PRG4 (Proteoglycan 4) ELISA Kit

This sandwich ELISA from Assay Genie is designed for the quantitative measurement of human proteoglycan 4 (PRG4) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit uses a double-antibody sandwich format with colorimetric detection. PRG4, also referred to by the synonyms lubricin, superficial zone proteoglycan (SZP), and megakaryocyte-stimulating factor, corresponds to UniProt entry Q92954 and is associated with signal transduction research.

The kit reports a sensitivity of 0.75 ng/mL and an assay range of 1.25–80 ng/mL. Kit components are stored at 2-8°C, with a shelf life of 12 months. Linearity was evaluated in serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, with reported linearity ranges of 85-99% to 88-105% for serum, 86-98% to 84-89% for EDTA plasma, and 82-96% to 86-99% for heparin plasma. Recovery averages are 95% for serum, 100% for EDTA plasma, and 96% for heparin plasma.

Proteoglycan 4 is described as a key protein involved in lubricating joints and protecting cartilage, making it essential for maintaining joint health and function. Abnormal PRG4 levels have been linked to osteoarthritis and rheumatoid arthritis, supporting its value for investigating joint disease and potential therapeutic interventions.