Human PRG3 (Proteoglycan 3) ELISA KIT (HUFI08100)

The Assay Genie quantitative immunoassay targets human proteoglycan 3 (PRG3), an eosinophil granule protein investigated in the context of asthma pathogenesis, eosinophilic esophagitis, and other allergic or helminth-driven disorders. Researchers profiling eosinophil activation markers can rely on this colorimetric ELISA kit to quantify PRG3 in prepared serum or plasma specimens harvested from human donors.

Reagents arrive as a complete microplate kit arranged for ninety-six individual measurements, with each antibody-coated well providing a capture surface for circulating PRG3. The kit includes calibrated standards for generating a calibration curve, biotinylated detection antibody, enzyme conjugate working solution, wash buffer concentrate, colorimetric substrate, and stop reagent, packaged in single-use vials sufficient for one full plate run. The protocol is performed entirely in vitro and is compatible with automated plate washers and standard absorbance microplate readers commonly found in immunology laboratories.

Refrigerated storage of unopened vials is recommended, and standard aliquoting of reconstituted reagents into smaller tubes helps preserve antibody activity across repeated freeze-thaw cycles. Internal quality controls supplied with the kit allow verification of inter-assay precision before committing limited clinical or research samples to the plate.