Human PREX2 ELISA Kit

A sandwich ELISA from Assay Genie is designed for the quantitative measurement of PREX2 in human samples. PREX2 participates in cell signaling, cell growth, and cell migration, and its dysregulation has been linked to cancer progression, making this assay a relevant tool for cancer research and cell biology studies.

Using a double-antibody, colorimetric format, the kit reports a sensitivity of 46.875 pg/mL and a working range of 78.125–5000 pg/mL. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with the kit stable at 2-8°C for 12 months. Supplied components include a pre-coated microplate, lyophilized standard, biotin-labeled detection antibody, HRP-streptavidin conjugate, TMB substrate, wash and dilution buffers, stop solution, plate sealer, and a technical manual. Linearity across serum, EDTA plasma, and heparin plasma (n=5) at 1:2, 1:4, and 1:8 dilutions returns values within 81–102%, while recovery averages 95% (serum), 94% (EDTA plasma), and 95% (heparin plasma).

The protocol follows a standard sandwich workflow: equilibrate reagents and TMB substrate, load standards and samples on the pre-coated plate, incubate at 37°C for 90 minutes, add biotin-labeled detection antibody for 60 minutes, incubate with HRP-streptavidin for 30 minutes, develop color with TMB in the dark for 10–20 minutes, and read absorbance at 450 nm after adding stop solution.