Human Prekallikrein (PK) ELISA Kit

Prekallikrein, also known as Fletcher factor, is the circulating zymogen precursor of plasma kallikrein that travels in plasma bound to high-molecular-weight kininogen. It sits at the entry point of the contact activation pathway, where activated Factor XII cleaves it into kallikrein, which in turn liberates bradykinin and feeds back to amplify surface-driven intrinsic coagulation. Measuring prekallikrein in human plasma is relevant to research on hereditary angioedema, contact system dysregulation, sepsis-related coagulopathy, and the kallikrein-kinin axis. This quantitative ELISA from Assay Genie is supplied as a complete kit in a microplate format and is intended for research use only with human plasma specimens. The protocol provides a standard curve generated from calibrated references, allowing measurement across a working range of unknowns, with all reagents, buffers, and detection components included for immediate use. Plasma samples are commonly processed according to standard citrate collection procedures, and the kit is intended for laboratory professionals studying hemostasis, complement crosstalk, or vascular inflammation. Each kit is configured as a single-use set of reagents for one full microplate run, with sufficient volume for both standards and samples in duplicate.