Human Pregnane X Receptor (PXR) ELISA Kit

This quantitative immunoassay from Assay Genie measures human Pregnane X Receptor (PXR), a ligand-activated nuclear receptor that governs transcription of CYP3A4 and related drug-metabolizing enzymes. PXR expression is widely studied in pharmacology, drug-drug interaction work, and toxicology, where quantifying receptor abundance in hepatocytes or intestinal cells helps researchers evaluate cellular response to chemical exposure.

Reagents are supplied for 96 assays in a microplate format with a colorimetric readout, letting labs use the standard plate readers already present in most life-science facilities. Sample preparation is straightforward: crude cell lysates prepared from cultured hepatocytes or homogenates from snap-frozen tissue can be loaded with minimal pre-processing, which preserves endogenous receptor levels during the quantification step.

Common applications include monitoring PXR induction after compound treatment in vitro, comparing baseline expression across donor-derived primary hepatocyte lots, and profiling receptor abundance in disease models where altered drug clearance is under investigation.