Human Pregnancy Associated Plasma Protein A (PAPPA) ELISA Kit

The Assay Genie human PAPPA enzyme immunoassay enables quantitative measurement of pregnancy-associated plasma protein A in human specimens using a two-antibody absorbance-based protocol completed in a 96-assay plate format. PAPPA is a zinc-binding metalloproteinase that cleaves insulin-like growth factor binding proteins and serves as a clinically validated biomarker in first-trimester aneuploidy screening, where low maternal values are associated with trisomy 21 and other chromosomal abnormalities. Beyond obstetric applications, circulating PAPPA has emerged as a research indicator of atherosclerotic plaque instability and acute coronary events, making this kit useful across reproductive medicine, cardiovascular investigation, and studies of preeclampsia, fetal growth restriction, and trophoblast biology. The assay accommodates multiple human-derived specimen types including tissue homogenates, cell lysates, and conditioned media from cultured cells, supporting both clinical sample analysis and basic in vitro research. Each kit contains the reagents required to generate a standard curve and analyze unknowns, with components intended to be equilibrated to room temperature before use and working standards aliquoted to limit freeze-thaw cycles. Prepared wells should be shielded from light during signal development to preserve measurement stability. The procedure is intended for research use only and is not validated for clinical diagnostic decision-making.