Human Prefoldin Subunit 2 (PFDN2) ELISA Kit

For cytoskeletal chaperone research, this Assay Genie kit enables quantitative measurement of Prefoldin Subunit 2 (PFDN2) in human specimens via a competitive ELISA workflow with colorimetric readout on a standard microplate. PFDN2 functions as part of the heterohexameric prefoldin chaperone complex, which captures unfolded cytoskeletal substrates such as actin and tubulin and delivers them to group II chaperonins for ATP-dependent folding.

Investigators apply this assay to tissue homogenates, cell lysates, and clarified biological samples from human donors to examine chaperone expression, cytoskeletal assembly dynamics, and protein homeostasis under varied experimental conditions. The kit provides a pre-coated plate, lyophilized standards, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent in individually sealed vials, with reagents sufficient for one full plate run.

Chromogenic development produces absorbance values proportional to PFDN2 concentration, and a standard curve generated from serial dilutions enables interpolation of unknown samples. For research use only, the workflow should be completed within the kit's stated shelf life, with unused components refrigerated and protected from light to preserve reagent activity.