Human Prealbumin/Transthyretin ELISA Kit

From Assay Genie, this Human Prealbumin/Transthyretin ELISA Kit is a sandwich, double-antibody immunoassay designed for the precise quantification of prealbumin/transthyretin in human samples. Prealbumin, also known as transthyretin, is a carrier protein that transports thyroid hormones and retinol (Vitamin A) in the blood and is associated with conditions including malnutrition, liver disease, and inflammation, making it a useful biomarker for studies of nutritional status, liver function, and carrier-protein biology.

The kit uses a colorimetric detection method and accepts a broad range of human sample types, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported performance characteristics include a sensitivity of 4.688 pg/mL, a measuring range of 7.813–500 pg/mL, and storage at 2–8°C for 12 months. Linearity has been validated across dilutions in serum, EDTA plasma, and heparin plasma (n = 5), and recovery averages 100% in serum, 95% in EDTA plasma, and 97% in heparin plasma. The workflow involves equilibrating reagents to room temperature, a primary incubation at 37°C for 90 minutes, biotinylated detection-antibody binding at 37°C for 60 minutes, HRP-streptavidin binding at 37°C for 30 minutes, TMB color development in the dark for 10–20 minutes, and absorbance reading at 450 nm after addition of the stop solution.