Human PPT2 ELISA Kit (HUFI07067)

Lysosomal thioesterases such as Palmitoyl-Protein Thioesterase 2 (PPT2) cleave thioester bonds that link fatty acyl groups to cysteine residues of S-acylated proteins, a reversible lipid modification that governs membrane association, vesicular trafficking, and protein turnover in many cell types. As a lysosomal hydrolase, PPT2 contributes to the catabolism of modified proteins delivered to the endolysosomal system and is implicated in cellular lipid homeostasis and immune cell signaling pathways, making quantitative measurement valuable in research on lysosomal biology and metabolic regulation.

The Human PPT2 ELISA Kit from Assay Genie provides a complete immunoassay platform for quantifying endogenous PPT2 protein in human research samples, with reagents supplied in a single pack of 96 Assays. The kit includes a pre-coated microplate, lyophilized recombinant PPT2 standards packaged in sealed vials, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, substrate solution, stop solution, and a printed protocol. Each assay uses a defined sample volume added to antibody-coated wells, followed by incubation, washing, and signal development through an enzymatic chromogenic reaction that is read on a standard microplate reader.

Researchers investigating PPT2 abundance in primary cells, patient-derived samples, or tissue lysates can apply this kit to compare expression across experimental conditions. All components are intended for research use only in laboratory settings, and refrigerated storage is recommended for opened reagents to preserve activity through the workflow.