Human PPP2R5A ELISA Kit (HUFI07087)

The Assay Genie Human PPP2R5A ELISA Kit provides quantitative measurement of the B56α regulatory subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase scaffold involved in Wnt/β-catenin signaling, Ras-Raf-MEK-ERK pathway attenuation, and G1/S cell cycle progression.

This assay supports research into tumor suppressor biology, leukemia signaling, kinase-phosphatase crosstalk, and PP2A subunit expression profiling. Investigators studying phosphatase deregulation in cancer, neurodegenerative disease models, or checkpoint control frequently combine B56α quantification with western blot validation to characterize PP2A holoenzyme composition across different cell types and tissue contexts.

Each kit supplies a pre-coated microplate, biotinylated detection antibody, recombinant protein standards, streptavidin-HRP conjugate, wash buffer, substrate, and stop solution for 96 individual determinations. The standard workflow uses 100 µL of sample per well and is intended for research use only in laboratory settings.