Human PPP1R1A ELISA Kit

Designed for quantitative detection of PPP1R1A (Protein Phosphatase 1 Regulatory Inhibitor Subunit 1A) in human samples, this Sandwich ELISA from Assay Genie employs a double-antibody, colorimetric workflow. The assay accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with read-out at 450 nm following TMB color development.

Reported performance characteristics include a sensitivity of 37.5 pg/mL and a standard range of 62.5-4000 pg/mL. Linearity has been evaluated at 1:2, 1:4, and 1:8 dilutions across serum (n=5): 85-97%, 85-97%, and 86-103%; EDTA plasma (n=5): 84-100%, 82-97%, and 84-100%; and heparin plasma (n=5): 82-99%, 81-91%, and 83-99%. Recovery ranges with averages are serum 89-103% (95%), EDTA plasma 90-105% (98%), and heparin plasma 85-98% (92%).

The kit is intended for research applications in metabolism, with storage at 2-8°C for 12 months. Associated identifiers listed by the manufacturer include Uniprot Q13522 and the synonyms Protein phosphatase inhibitor 1, I-1, and IPP-1.