Human PPP1CA/Serine/threonine-protein phosphatase PP1-alpha catalytic subunit ELISA Kit

A colorimetric Sandwich ELISA from Assay Genie designed for the quantitative measurement of human PPP1CA (Serine/Threonine Protein Phosphatase PP1 Alpha Catalytic Subunit) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

The assay reports a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL. Storage is specified at 2–8°C for 12 months. PPP1CA is described as a critical enzyme regulating cellular processes through dephosphorylation of target proteins, with roles in cell division, signal transduction, and gene expression; its dysregulation has been linked to cancer and neurological disorders, supporting its value as a biomarker for disease research and therapeutic investigation.

Reported linearity across 1:2, 1:4 and 1:8 dilutions: serum (n=5) 87-94%, 86-105%, 90-104%; EDTA plasma (n=5) 82-94%, 89-101%, 92-100%; heparin plasma (n=5) 84-95%, 81-96%, 81-100%. Reported recovery: serum (n=5) 85-96% average 91%; EDTA plasma (n=5) 85-103% average 96%; heparin plasma (n=5) 90-97% average 94%. The procedure involves equilibrating reagents, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding, HRP-Streptavidin incubation, TMB substrate development in the dark for 10–20 minutes, and absorbance reading at 450 nm.