Human PPM1L ELISA Kit (HUFI07102)

The Human PPM1L ELISA Kit from Assay Genie is configured for quantitative measurement of Protein Phosphatase, Mg2+/Mn2+ Dependent 1L (PPM1L), a magnesium-dependent serine/threonine phosphatase implicated in cellular stress responses, ER stress signaling, and regulation of the Smad pathway. Researchers use the assay to profile PPM1L expression in human serum and plasma specimens, supporting studies in inflammation biology, metabolic disease, and intracellular signal transduction.

Each 96-well microplate is supplied with a pre-coated capture antibody, biotinylated detection reagent, streptavidin-HRP conjugate, recombinant standards, and chromogenic substrate. The protocol follows a standard colorimetric workflow with absorbance read at 450 nm. A typical 100 uL sample volume is required per well, and the capture antibody is calibrated for human-specific binding, with no significant cross-reactivity reported against rodent PPM1L orthologues. Reconstituted reagents should be stored at 4 degrees C for short-term use and at -20 degrees C for long-term stability, protected from light to preserve enzymatic activity.

Typical research applications include quantitative biomarker profiling, pathway-level analysis, and treatment-versus-control comparisons across patient cohorts. The 96 Assays plate configuration supports full standard curves plus replicate wells for unknown samples within a single experimental run.