Human PPID ELISA Kit (HUFI07106)

Quantify human Cyclophilin D (peptidyl-prolyl isomerase D, encoded by the PPID gene) with this matched antibody pair ELISA from Assay Genie, formatted as a 96 Assays research kit. The assay is intended for measurement of endogenous PPID in laboratory samples and supports work on mitochondrial permeability transition, calcium homeostasis, and apoptosis signaling.

Cyclophilin D localizes to the mitochondrial matrix where it modulates the permeability transition pore (PTP), a central effector in necrosis, ischemia-reperfusion injury, and cardioprotection research. The plate-based immunoassay uses a capture antibody immobilized on a microplate and a detection antibody producing an absorbance readout after substrate conversion, compatible with standard ELISA plate readers.

Kit contents include a pre-coated microplate, matched antibodies, recombinant reference standard, and buffer reagents sufficient for 96 Assays. Reagents are stored at 4 degrees C for short-term use; aliquoted components are stable at -20 degrees C for longer periods. Useful downstream workflows include western blot validation, cell culture lysate profiling, and immunohistochemistry follow-up on fixed tissues.