Human PPCS ELISA Kit (HUFI07095)

The Human PPCS ELISA Kit from Assay Genie quantifies endogenous phosphopantothenoylcysteine synthetase, the cytosolic enzyme catalyzing the second committed step of coenzyme A biosynthesis. By converting 4'-phosphopantothenate to 4'-phosphopantothenoylcysteine in an ATP-dependent reaction, PPCS sits at a critical branch point linking pantothenate uptake to the universal CoA cofactor pool, making this microplate-based immunoassay useful for studies of metabolic flux, mitochondrial function, and cellular energetics.

The standard protocol uses 100 µL of sample per well, with absorbance read on a standard plate reader. Each kit includes pre-coated wells, biotinylated detection antibody, recombinant human PPCS standards, streptavidin-HRP, wash buffer, substrate, and stop solution. Reconstituted components should be refrigerated and protected from light, and the kit is intended for in vitro research use only. Human blood-derived specimen types support accurate endogenous PPCS quantification across the standard curve.