Human PPAR-gamma (Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit (HUES02406)

A colorimetric Sandwich ELISA kit from Assay Genie is designed for the accurate and reliable detection of PPAR Gamma levels in human samples, including serum, plasma, and other biological fluids. The assay provides precise, reproducible results suited to a range of research applications. PPAR Gamma is a key transcription factor involved in the regulation of lipid metabolism, insulin sensitivity, and inflammatory responses, and its dysregulation has been linked to obesity, diabetes, and cardiovascular disorders.

The kit uses a Sandwich-ELISA principle on a pre-coated microplate. Standards or samples bind to an immobilized antibody, followed by a biotinylated detection antibody and an Avidin-Horseradish Peroxidase (HRP) conjugate. After substrate addition, colour development is measured at 450 nm ± 2 nm and the target concentration is determined from a standard curve. Sensitivity is 0.1 ng/mL, with a detection range of 0.16-10 ng/mL and reported recovery of 80%-120%. Total assay time is 3 h 30 min. The kit recognizes Human PPAR-γ in samples, with no significant cross-reactivity or interference observed between the target and analogues.

Relevant research areas include cancer, cardiovascular biology, epigenetics and nuclear signaling, metabolism, neuroscience, and stem cells. UniProt ID: P37231. Sample volume: 100 μL. Storage: 2-8°C.